人细胞因子抗体芯片10

更新:2014/7/23 10:49:54      点击:2255
  • 产品型号   AAH-CYT-G10
  • 产品描述

    Raybiotech Products

产品介绍

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information

4-1BB
(TNFRSF9/CD137)
ACE-2
Alpha-fetoprotein
Angiopoietin-1
Angiostatin
ANGPTL4
Beta-2 Microglobulin
BCMA
(TNFRSF17)
beta IG-H3
CA125
CA15-3
CA19-9
CA9
Cathepsin S
HCC-1
(CCL14)
6Ckine
(CCL21)
CD23
CD40 Ligand
(TNFSF5)
CEA
CEACAM-1
Cripto-1
CRP
(C-Reactive Protein)
DAN
Decorin
DKK-1
Dkk-3
Dkk-4
CD26
(DPPIV)
E-Cadherin
EDA-A2
EG-VEGF
(PK1)
TROP1
(EpCAM)
ErbB2
Erythropoietin R
FSH
HB-EGF
hCG intact
HVEM
(TNFRSF14)
IL-13 R1
IL-17B
IL-17C
IL-17F
IL-17 RA
Procalcitonin
PSA-free
S100 B
Sonic Hedgehog N-Terminal
(Shh-N)
Thyroglobulin
Ubiquitin+1

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array G10 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Number of Targets Detected

49

 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
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