人细胞因子抗体芯片5

更新:2014/7/23 10:38:56      点击:2253
  • 产品型号   AAH-CYT-G5
  • 产品描述

    Raybiotech Products

产品介绍

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information

ENA-78
(CXCL5)
GCSF
GM-CSF
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
I-309
(TCA-3/CCL1)
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-3
IL-4
IL-5
Angiostatin
IL-7
IL-8
(CXCL8)
IL-10
IL-12 p40/p70
IL-13
IL-15
IFN-gamma
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
M-CSF
MDC
(CCL22)
MIG
(CXCL9)
MIP-1 beta
(CCL4)
MIP-1 delta
(CCL15)
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
TARC
(CCL17)
TGF beta 1
TNF alpha
TNF beta
(TNFSF1B)
EGF
IGF-1
Angiogenin
Oncostatin M
Thrombopoietin
(TPO)
VEGF-A
PDGF-BB
Leptin
BDNF
BLC
(CXCL13)
Ck beta 8-1
(CCL23)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
FGF-4
FGF-6
FGF-7
(KGF)
FGF-9
Flt-3 Ligand
Fractalkine
(CX3CL1)
GCP-2
(CXCL6)
GDNF
HGF
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IL-16
IP-10
(CXCL10)
LIF
Light
(TNFSF14)
MCP-4
(CCL13)
MIF
MIP-3 alpha
(CCL20)
NAP-2
(PPBP/CXCL7)
NT-3
NT-4
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
PARC
(CCL18)
PLGF
TGF beta 2
TGF beta 3
TIMP-1
TIMP-2

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array G5 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Number of Targets Detected

80

 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
 
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