小鼠细胞因子抗体芯片5

更新:2014/7/21 17:26:37      点击:2112
  • 产品型号   AAM-CYT-5
  • 产品描述

    Raybiotech Products

产品介绍

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information

4-1BB
(TNFRSF9/CD137)
6Ckine
(CCL21)
ACE
ALK-1
Amphiregulin
Cardiotrophin-1
(CT-1)
CD27
(TNFRSF7)
CD27 Ligand
(TNFSF7)
CD36
(SR-B3)
CD40 Ligand
(TNFSF5)
Chordin
CTLA-4
(CD152)
Decorin
DKK-1
E-Cadherin
EGF
Endoglin
(CD105)
Epigen
Epiregulin
Galectin-1
Gas 1
Gas 6
GITR Ligand
(TNFSF18)
Granzyme B
HAI-1
HGF
IL-1 R4
(ST2)
IL-11
IL-17B
IL-17E
(IL-25)
IL-17F
IL-1 RA
(IL-1 F3)
IL-2 R alpha
IL-20
IL-21
IL-28A
(IFN-lambda 2)
IL-6 R
JAM-A
(CD321/F11R)
MAdCAM-1
MFG-E8
Neprilysin
Pentraxin-3
(TSG-14)
Prolactin
RAGE
TACI
(TNFRSF13B)
TREM-1
TWEAK
(TNFSF12)
TWEAK R
(TNFRSF12)

Species Detected

Mouse

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C5 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Number of Targets Detected

48

 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
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